Journal: Emerging Microbes & Infections

Article Title: Multidrug-resistant tuberculosis (MDR-TB) strain infection in macaques results in high bacilli burdens in airways, driving broad innate/adaptive immune responses

doi: 10.1038/s41426-018-0213-z

Figure Lengend Snippet: a Representative flow cytometry data (left) show percentages of CD3+ T effector cells producing IFN-γ (upper panel) and perforin in PBMCs from MDR-Mtb- and Erdman-infected macaques at weeks 3 and 6, respectively. Data are represented as the mean ± SEM. b Representative flow cytometry data (left) show percentages of CD3+ T effector cells producing IL-17 (upper panel) or IL-22 in PBMCs at weeks 3 and 6, respectively. Data are represented as the mean ± SEM. c The data represent the mean percentages ± SEM of CD3+ T effector cells producing IFN-γ and perforin, TNF-α, IL-17, and IL-22 in BALF at week 6. d The data show the mean numbers or percentages ± SEM of CD4+ CD25+ Foxp3+ T cells in blood and BAL and those of CD8+ CD25+ Foxp3+ T cells in BAL at weeks 3 and 6 after infection. e Data are represented as the mean numbers or percentages ± SEM of CD8+ IFN-γ+ T cells in blood and BALF measured by ICS with or without PPD stimulation at different time points. Similar data trends for CD4+ IFN-γ+ T cells in blood and BALF measured by ICS with or without PPD stimulation (data not shown). Data were derived from up to six MDR-Mtb-infected and nine Erdman-infected macaques and were analyzed by the Mann–Whitney test (nonparametric method). * p < 0.05, ** p < 0.01

Article Snippet: The following antibodies were used for culturing or surface and intracellular cytokine staining (ICS) for flow cytometry: CD28 (CD28.2, BD), CD49d (9F10, BD), CD3-PB (SP34-2, BD), CD4-BV510 (L200, BD), CD8-PB (RPA-T8, BD), IFN-γ-APC (4S.B3, BD), IFN-γ Brilliant Violet 711 (4S.B3, Biolegend), TNF-α-PE (MAb11, BD), TNF-α-PE-Cy7 (MAb11, BD), IL-17-PE (eBio64CAP17, eBioscience), IL-22-biotinylated (anti-human IL-22, RD), Streptavidin-Pacific blue (Invitrogen), Perforin-biotinylated (Pf-344, Mabtech), Caspase 3-AF647 (C92-605,BD), and anti-Vγ2-FITC (7A5, Pierce).

Techniques: Flow Cytometry, Infection, Derivative Assay, MANN-WHITNEY